Microplate luminescent reader assay for ERK activity. The Multiskan Sky microplate reader is an absorbance plate reader. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). As a result, assay flexibility is consequence-free. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. New SpectraMax iD5 Multi-mode Microplate Reader. Optimized for fluorescence microplate readers. 1. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. The EMax Plus reader measures flat and round 96-well microplates. 9. #SpectraMax L Config) Methods. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 2 OD at 200 nm. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. i3x Multi-Mode Microplate Reader. No additional manuals are available. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. absorbance, fluorescence, and luminescence with the added functionality of. to each well. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. M3-M5e_manual. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. 14: Average %CV. ± 0. The procedure enables direct translation of any published equation. 03 pM 384-well: 0. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Abstract and Figures. 1/17 1907B Printed in USA . (FP) configured microplate readers. Dynamic range > 9 orders of magnitude. A. (12) Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility Nature CommunicationsDecember 1, 2022 Yukari Endo, Linda Groom, Alper Celik, Natalia. #I2511) • Assay diluent – may be cell culture medium. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Compare specifications and request a quote from leading suppliers. (FP) configured microplate readers. 2. Omnia® Compatible Microplate Reader Documentation Version No. To confirm the accurate positioning of arrayed colonies, we performed an absorbance scan in a 96-well scheme with 30 × 30 scans per well for an OmniTray agar plate with 96. M3-M5e_manual. Next generation SpectraMax® absorbance microplate readers. 0. Four wash/rinse bottles are included and configurable for both 96- and 384. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. . tools to expand the boundaries of their research. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. 585The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. best-in-class data . Add 150 µL reagent to each well. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. Reading a Microplate . The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Plates can put in the drawer both in landscape and portrait positions. Key applications: ELISA, nucleic acid, endotoxin testing. Minneapolis, MN, USA), according to the manufacturer's protocol. BSA concentration (µg/mL) Average OD. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. 2 2601A ValitaTiter. CloneMatrix. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. Table 1: Categories of heating element design. MycoAlert Reagent was added to each well and incubated at room temperature for 5. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. 3. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. white 96 well plate and 75. 2 mm x 86 mm) Height: between 13. Make sure to Data 96孔微孔板的数据对比. Temperature control. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Four wash/rinse bottles are included and configurable for both 96- and 384. Our microplate. 2 W x 44. In addition, the iD5 reader measures TRF and FP and. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. section, select plate section titled. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Three low volume 24-well bottom slides. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. microplate reader (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc manufactures this product . the appropriate well of the microplate. 1 OD at 230 nm. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. (Molecular Devices cat. ©2010 molecular Devices, inc. Figure 2-23: Bottle holder To install the bottle holder: 1. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. microplate . Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. This reader provides visible. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). Instrument settings are listed in Table 1. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. With various on-board features and capabilities,. • 0. ©2017 Molecular Devices, LLC . All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. I have used Molecular Devices’ Vmax 96-well microplate readers since about the time of their inception in the mid to late-1980s. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. Read the microplate Step 1: Place the microplate in the reader. Integrated stacker option. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. 96-well: 0. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. VANTAstar™ Flexible Multi-mode Microplate Reader. Measure more accurately. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Built in near field communication (NFC). A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. In addition, SoftMax Pro Software In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. We would like to show you a description here but the site won’t allow us. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. #iD5-STD) Methods ATP standard curve. In simplest terms, a plate reader measures and. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Fluorescence Microplate Reader Comparison Chart. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Visible absorbance -. SpectraMax 250 User. Create custom fluorescence assays without having to purchase expensive filter sets. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. 300 µL of Detergent Compatible Bradford reagent was added to each well. Use the provided 3. Top or bottom detection is available with a simple click of a button. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Materials. # 0310-5218T). The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. Comparing Luminescence sensitivity of SpectraMax Microplate Readers using ATP Assay | Molecular Devices Author: Molecular DevicesMake the most of your budget with the VersaMax™ ELISA Microplate Reader, our affordable, visible spectrum absorbance reader for 96-well microplates. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. 384-well: 0. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. 984. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Molecular Devices VersaMax Microplate Reader Specifications. #ID3-STD) • L-Tryptophan (Sigma cat. A control well was set up to verify. ValitaTiter Plus 96-well (1 plate) VAL004. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Industry standard, 96-well plates are. Growth medium was then replaced with fatty acid uptake solution. These robust readers have been placed in labs from. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . , 384 wells < 45 sec. Photometric and kinetic analysis mode. Cross-talk < 0. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. This . 03 pM. The system provides options for 20-, 40-,. An example of dual-luciferase assay results. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. #W50-100). SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax iD5. SpectraDrop High-Throughput Screening (HTS) Kit. Five preferred modes for diverse bioresearch applications. #L6876) Guanidine hydrochloride (Sigma cat. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. 03 pM 384-well: 0. (Figure 1). 6 L (with SpectraMax i3x System) Title: SpectraMax MiniMax 300 Imaging Cytometer | Molecular. Description. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. We would like to show you a description here but the site won’t allow us. 7°C (gradient heating design). 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Outer wells are aligned for proper viewing with FLIPR. U-bottom: 30-40 µl. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 3. #3693; n = 4 for each standard). MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. , Sigma cat. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. Compact and tunable absorbance microplate readers DATA SHEET. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Eight filter modes enable applications such as protein quantification, cell viability and ELISA. Find a new or used microplate reader and lab equipment for sale and auction at LabX. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Or scan up to 384 wells at any time to determine the best assay conditions. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. 300 µL of Detergent Compatible Bradford reagent was added to each well. Published Dec 16, 2020 | Updated Dec 16, 2020. 100 fmol 1536-well (8 µL) 0. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. 375 pM. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Reactions catalyzed by firefly and *Renilla luciferases. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Or scan up to 384 wells at any time to determine the best assay conditions. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. in a 96-well microplate. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Related Products: Low Binding Plates. personalized as your research. Wide range of applications The VersaMax reader covers. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Detergent Compatible Bradford assay. 5 mm and 15. the iD5 reader’s filter system. 1. Condition: Used - Good. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Microplate is validated by molecular devices for the FLIPR reader. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. Supplementary Information. Softmax Pro GxP Software. 384 well polystyrene microplates have plate dimensions of 127. Standard SBS 96 and 384 well plates (128. Other features and benefits include: Eliminate changing out filters. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Open the catalog to page 5. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Application Area: Spectrophotometer for molecular biology. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Molecular Devices' microplate readers. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. style. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. 76 L x 85. Wavelength Selection. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. for ValitaTiter Plus standards read on Molecular Devices readers. HPV Serology Laboratory Standard Operating Procedure . a white 96-well half-area microplate (Corning cat. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Specifications for the SpectraMax L microplate reader. Deep well blocks available in 24, 96 and 384-well formatsFlexStation® 3 Multi-Mode Microplate Readers . The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Elisa Plate Readers and Microplate Readers at Biocompare. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. 003 fmol 0. tecHnical SPecificationS Performance Specifications Measurement range: 400–750 nm 0–4. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. The CellTiter-Glo assay from Promega uses luciferase enzyme. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. It also integrates with the powerful SoftMax Pro Software for. It also allows for the elimination of standard curves. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. 98 Bovine serum albumin mg/mL O. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. An industry-renowned instrument for monitoring GPCRs and ion channels. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). Affordable multi-mode microplate reader with . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Step 3: Click “Settings” and a screen will appear (Figure 1). 96-well microplate at 200 µL/well. 3. 6. absorbance, fluorescence, and luminescence with the added functionality of. Endpoint, kinetic, spectrum. , Sigma cat. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. 020 fmol 0. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. This reader's dual monochromators for. Running both standard spectrophotometer and microplate. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. These instruments are all elegant and simple in their form and function. Plate Formats: 6 to 384-well microplates. 96-well flat clear bottom white TC-treated microplates (Corning cat. 14: Average %CV. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. Efficient cell washing with minimal cell loss and optimal assay performance. 5°C at 37°C. Fluorescence Microplate Reader Comparison Chart. Setup Guide. The drawing shows a standard microplate of 15 mm height covered with a lid. Learn more about how absorbance is measured, and some key applications that utilize. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. Microplate readers use a vertical light path so the distance of the. Five-second read per plate. Compact design Minimum volume and. Keywords: fluorescence polarization, trf, fret, igg. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Wavelength Range 200 to 999 nm. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. Materials. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aThe Molecular Devices Aquamax 4000 With 96 Well Head AQUAMAX 4096-G is a quality addition to the Molecular Devices lineup. 2 OD at 200 nm; this decreased to 0. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Molecular Devices provides. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the. 1536-well: 0.